International regulation of food microorganisms


The food business has continuously been one in all the foremost necessary market segments, as its main task is to satisfy basic needs. The most feature of this field of activity is that the product made by it are in direct contact with the body of the top consumer. Thus, the presence of unsafe micro-organisms in foodstuffs can cause significanT hurt to health. Of course, in drawing these conclusions, we tend to take under consideration not solely food for people, however conjointly food for animals. Animal feed should, within the same manner as food for humans, endure analysis to verify their safety for the body. it's particularly value accenting the importance of analyzing nutrients, supported the large form of microorganisms that, for one reason or another, can get into their composition. Every microorganism, microbiotic strain or different substance which will be found in food can create a separate potential threat. Therefore, each type of food product must undergo its own multi-level verification. So as to unify these analyzes, yet on guarantee safety and quality, international standards are being created that are perpetually updated taking under consideration new microbiological discoveries. The data that we've ready for you during this article can assist you increase your power within the market, yet as strengthen your position.


Animal feeding stuffs: strategies of sampling and analysis - Detection and enumeration of baker's yeast used as feed additive

EN 15789:2021


Probiotics are food supplements that facilitate to normalize the enteral microflora of animals, as well as maintain the health of the body as a whole. Despite the helpful properties of probiotics, their presence and content in animal feed products ought to be controlled and strictly dosed. It should be noted that there are a large variety of types of probiotic strains, that are baker's yeast, the analysis method of which is delineated within the international standard, resembling EN 15789:2021.


This document specifies general rules for the enumeration of fungus genus cerevisiae in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain Saccharomyces cerevisiae as one organism element or in a mixture with different microorganisms. Applying the strategy to premixtures and compound feeds with essential amounts of copper demands a special procedure (see Annex A). The document isn't applicable to mineral feeds, that are outlined as complementary feeding stuffs composed principally of minerals and containing a minimum of forty p.c crude ash (Regulation (EC) 767/2009) [3].

There are totally different classes of feed samples:

a) Additives containing concerning 1010 colony forming units (CFU)/g; b) Premixtures containing about 1011 CFU/kg; c) Compound feeds, meal or pellets which contain about 109 CFU/kg.


The helpful properties of probiotics have long been a well-tried truth. The presence of those organic process parts in animal feed is that the key to the health of the animal, which, in turn, is the main goal of entrepreneurs who are engaged in animal husbandry. The assembly of animal feed should be subject to strict regulations, therefore we tend to advise you to inform yourself with the text of this customary if you would like to be a pacesetter in your segment.


Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - half 1: technique mistreatment quick alkaline transesterification and mensuration for 3-MCPD and differential measurement for glycidol

EN ISO 18363-1:2021


The presence of glycidyl ethers in food can, below sure conditions, be harmful to the human body, so this indicator must be controlled in accordance with international quality and safety customarys. Given the potential hazard, product resembling oils and fats should be tested in order that the manufacturer will offer a guarantee of their safety. One in all the analysis methodologies is delineated in a world standard such as lEN ISO 18363-1:2021.


This a part of ISO 18363 describes a procedure for the indirect determination of 3-MCPD organic compound (bound 3-MCPD) and doable free 3-MCPD when alkaline catalysed ester cleavage and derivatization with phenylboronic acid (PBA). Furthermore, this part of ISO 18363 permits the indirect determination of glycidyl esters (bound glycidol) below the assumption that no different substances are gift that react at temperature with inorganic chloride to come up with 3-MCPD. This a part of ISO 18363 is applicable to therefore lid and liquid fats and oils. Milk and milk product (or fat returning from milk and milk products) are excluded from the scope of this part of ISO 18363.


We tend to advise you to listen to the scope of this standard, namely, that its necessities don't apply to farm products, so if your company is engaged in production during this industry, then you ought to pay attention to other documents. However, if you're engaged within the production of animal and vegetable fats and oils, then it'll be helpful for you to implement this data into your work.


Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - half 3: technique mistreatment acid transesterification and mensuration for 2-MCPD, 3-MCPD and glycidol

EN ISO 18363-3:2021


Foods that contain fatty acids may additionally contain substances which will hurt the human body. Among such substances, we are able to embrace chloropropanediols (MCPD) and glycide. it's these substances in the composition of foods that can provoke fertility problems, therefore it's value rigorously watching their presence. a world customary has been created for this purpose resembling EN ISO 18363-3:2021.


This document specifies a procedure for the synchronous determination of 2-MCPD organic compounds (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a very single assay, supported acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) before GC/MS analysis.

This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and also the limit of detection (LOD) is 0,03 mg/kg.

A feature of the international standard that we tend to mentioned higher than is that the data in it may be applied to solid and liquid fats and oils, that successively makes it terribly helpful for firms that manufacture these product. You'll browse the total text of the quality on our website.


Biology of the organic phenomenon - enzyme chain reaction (PCR) for the detection of microorganisms - Thermal performance testing of thermal cyclers

EN ISO 20836:2021


Before being on the counter, and soon the consumer' table, food products must undergo a multi-level verification method so as to exclude the presence of unsafe microorganisms for the human body. Given the high importance of this analysis, it becomes obvious however necessary it's to possess the correct instrumentation for these purposes. One in all the required equipment are thermal cyclers, that successively have variety of important characteristics. All necessities for the employment of thermal cyclers are delineated within the international standard EN ISO 20836:2021.


This document specifies requirements for the installation, maintenance, temperature standardisation and temperature performance testing of ordinary thermal cyclers and period thermal cyclers. It is applicable to the detection of microorganisms yet as the other applications within the organic phenomenon mistreatment enzyme chain reaction (PCR)-based methods. This document has been established for food testing, however is additionally applicable to other domains using thermal cyclers (e.g. environmental, human health, animal health, rhetorical testing).


Thermal cyclers are wide utilized in business to check food product for the presence of microorganisms. However, it's also value noting that this instrumentation may be used in other industries resembling care and environmental protection. Significantly noteworthy is that the undeniable fact that this International customary can be used for these areas too. Given the wide selection of uses for this document, we tend to encourage you to browse it in its completeness on our website.


Animal feeding stuffs: strategies of sampling and analysis - Detection and enumeration of Enterococcus (E. faecium) spp. used as feed additive

EN 15788:2021


Carboxylic acid microorganism of the genus Enterococcus (E. faecium) is a vital element of the healthy enteral microflora of mammals. Supported this, their presence in animal feed becomes a condition for the right functioning of the intestinal tract of animals. However, like different probiotics, they will solely offer edges to the body if they're in sufficient, controlled amounts. So as to regulate the amount of those organisms in a very animal feed, a world customary has been created EN 15788:2021.


This document specifies general rules for the enumeration of enterococci (E. faecium) in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain enterococci as one microorganism element or in a mixture with different microorganisms. Applying the strategy to premixtures and compound feeds with essential amounts of copper demands a special procedure (see A.2). The document isn't applicable to mineral feeds that are outlined as complementary feeding stuffs composed principally of minerals and containing a minimum of forty p.c crude ash (Regulation (EC) 767/2009) [4].

There are totally different classes of feed samples: a) Additives containing about 1010 colony forming units (CFU)/g; b) Premixtures containing 1011 CFU/kg; c) Compound feeds, meal or pellets that contain about 109 CFU/kg.


There are differing types of animal feed, and supported this classification, every of them has its own allowable content of carboxylic acid microorganism of the genus Enterococcus (E. faecium). In turn, these rules force makers of this product to rigorously management the assembly process. If your company is fascinated by manufacturing a high quality product in accordance with international standards, you ought to listen to the current international standard.


The necessity to research food product for the presence of risky constituents


Food production is invariably related to the danger of contamination with microorganisms which will be dangerous to the human body, or animals within the case of animal feed. Therefore, the challenge for producing firms is to make sure the protection of their products. To realize this confidence, it's necessary to hold out multi-level testing of food products before they reach the ultimate consumer. The majority of problems lies in the undeniable fact that there are so many microorganisms, and so every individual species should be tested singly mistreatment the suitable analysis technology. Given the very fact that food testing could need many totally different methods, we tend to anticipate that you just might have recommendation in choosing the appropriate International Standards for your company. Therefore, if your company is fascinated by increasing profits and increasing its competitive position within the market, then you'll look for advice from our specialists.


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